This product is distributed for laboratory research only. One unit is defined as the amount of Klenow Fragment (3’→5’ Exo-) that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37☌ using poly(dA-dT):poly(dA-dT) as a template:primer. Site-directed DNA mutagenesis using synthetic oligonucleotidesĢ5 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol.Generate single-stranded DNA probes using random primers The constructs are made blunt ended by filling in the single stranded overhangs using the Klenow fragment of E. Klenow Fragment is the Large Fragment of DNA Polymerase I, E.coli.Dideoxy DNA sequencing of single- or double-stranded DNA templates.Klenow-like fragment from Bacillus stearothermophilus, Gene, vol. Applications DNA blunting by fill-in of 5’-overhangs or removal of 3‘-overhangs. It exhibits 5'3' polymerase activity and 3'5' exonuclease (proofreading) activity, but lacks 5'3' exonuclease activity of DNA Polymerase I. coli DNA Polymerase I which retains polymerase activity but lacks 5’→3’ exonuclease activity and has mutations that effectively abolish the inherent 3’→5’ exonuclease activity. This plasmid (5.6-kb) was digested by PstI followed by exonuclease (Klenow fragment of DNA polymerase I) to remove a portion of the gene for -lactamase. The mutation D540E does not affect the DNA polymerase activity of the enzyme. Klenow Fragment is the Large Fragment of DNA Polymerase I, E.coli. Klenow Fragment (3’→5’ Exo-) is the large fragment of E.
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